No, please upload a single payroll register for each pay period.
To submit multiple pay periods, upload a separate payroll register file for each period in the 'Contributions' tab of the Employer Dashboard. Ensure you change the pay period start and end dates for each upload. This step must be performed for every payroll cycle. If there have been no changes since your last payroll upload, you can download the previously submitted file, update the file name if necessary, and submit it with the correct pay period dates.